Breckland Scientific 2021 Catalogue

Gauzes - Genetic & DNA

Gauzes

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1 CERAMIC CENTRE GAUZES - STANDARD UKAS Certified asbestos-free gauzes with ceramic centre. Cat No. Description Size Per 1 - 4 5+ GAU-100-125 Gauzes, Iron Wire, c/c 125 x 125mm 5 5.33 4.53 GAU-100-150 Gauzes, Iron Wire, c/c 150 x 150mm 5 6.78 6.10

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2 PLAIN GAUZES - STANDARD Plain iron wire gauzes. Cat No. Description Size

Price Per

GAU-050-125 Gauzes, Iron Wire

125 x 125mm

5.46 10

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GAU-050-150 Gauzes, Iron Wire

150 x 150mm

6.68 10

Gauzes, Ceramic Centre

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STAINLESS STEEL GAUZES - NEW! These stainless steel gauzes offer far better resistance to oxidisation and corrosion, leading to increased longevity. Cat No. Description Size Price Per GAU-300-125 Stainless Steel Gauzes 125 x 125mm 18.10 10 GAU-300-150 Stainless Steel Gauzes 150 x 150mm 21.91 10

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Related Products... For bunsen burners see page 107 If you need tripods see page 469 If you need bench mats see page 282

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Gauzes, Plain

Genetics

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3 TRANSFORMATION WITH GREEN FLUORESCENT PROTEINS In this experiment, students will explore the biological process of bacterial transformation using E. coli and plasmid DNA. At the end of the activity, students will have experience observing and analysing acquired traits (ampicillin resistance and fluorescence) as exhibited by transformed bacterial cells. Kit includes: instructions, BactoBeads™ E. coli GFP Host, supercoiled pFluoroGreen™ plasmid DNA, ampicillin, IPTG, CaCl 2 , Growth Additive, ReadyPour™ Luria Broth Agar (sterile), Luria Broth Medium for Recovery (sterile), petri plates (small), petri plates (large), plastic microtipped transfer pipettes, wrapped 10ml pipette (sterile), toothpicks (sterile), inoculating loops (sterile), microcentrifuge tubes All you need: automatic micropipette (5-50µl) and tips, two waterbaths (37°C and 42°C), thermometer, incubation oven (37°C), pipette pumps or bulbs, ice, marking pens, Bunsen burner, hot plate or microwave, hot gloves, long wave UV light. For 10 Lab Groups. Set up 50 minutes, Incubation overnight, Transform 15 minutes. Storage: Some components require refrigerator and freezer storage Cat No. Description Price Per BIO-900-200 Green Fluorescent Proteins 121.60 ea. 4 TRANSFORMATION WITH GREEN & BLUE FLUORESCENT PROTEINS The Green Fluorescent Protein (GFP), which is responsible for bioluminescence in the jellyfish Aequorea victoria, is used extensively in all areas of science. Many organisms have been transformed with the GFP gene. It has proven to be so useful that scientists have mutated it to produce Blue Fluorescent Protein (BFP). In this simple experiment your students will transform bacteria either with GFP, BFP or both! Kit includes: instructions, BactoBeads™, plasmid DNA, IPTG, ampicillin, transformation solution, ReadyPour™ agar, Luria broth, petri dishes, sterile pipets and loops. All you need: waterbath, 37¼°C incubation oven, microwave or hot plate, long wave UV lamp. For 10 Lab Groups. Complete in 50 minutes and grow overnight Storage: Some components require refrigerator and freezer storage Cat No. Description Price Per

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Transformation using Fluorescent Proteins

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Related Products... For reagents, biologicals and supplies see page189 For electrophoresis tanks see pages 186 - 187 For protein electrophoresis see page 373

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BIO-900-150 Green & Blue Fluorescent Proteins

148.00 ea.

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